منابع مشابه
Immunoblot analysis.
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. It involves the solubilization and electrophoretic separation of proteins, glycoproteins, or lipopolysaccharides by gel electrophoresis, followed by quantitative transfer and irreversible binding to...
متن کاملImmunoblot analysis of serological responses in invasive aspergillosis.
The serological response to Aspergillus fumigatus was investigated in patients with pulmonary aspergillosis using the immunoblot technique. Antibody was detected against nine components of the fungus, ranging in molecular weight from 88 000 to 33 000 daltons. Antibody to a 40 000 dalton component was present in most patients (13 of 16). In surviving patients it was high (two with aspergilloma) ...
متن کاملSonicated diagnostic immunoblot for bartonellosis.
Two simple Bartonella bacilliformis immunoblot preparation methods were developed. Antigen was prepared by two different methods: sonication of whole organisms or glycine extraction. Both methods were then tested for sensitivity and specificity. Well-defined control sera were utilized in the development of these diagnostic immunoblots, and possible cross-reactions were thoroughly examined. Sera...
متن کاملImmunoblot detection of antigens in immunoprecipitates.
Co-immunoprecipitation of antigens from cell or tissue extracts is used in a wide variety of experimental systems to corroborate the existence of authentic protein-protein interactions in vivo. Typically, stable protein-protein associations are detected by immunoprecipitation with antibodies specific for one protein, followed by immunoblot analysis with antibodies against potential interacting ...
متن کاملConsiderations when quantitating protein abundance by immunoblot.
The development of the immunoblot to detect and characterize a protein with an antisera, even in a crude mixture, was a breakthrough with wide-ranging and unpredictable applications across physiology and medicine. Initially, this technique was viewed as a tool for qualitative, not quantitative, analyses of proteins because of the high number of variables between sample preparation and detection...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2008
ISSN: 1940-087X
DOI: 10.3791/759